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class="clear"></div> </div> </div>  <script src="http://www.chem17.com/mystat.aspx?u=sunde17"></script>  <div id="bd8lmiie" class="ly_crumb"> <img src="/skins/342860/images/crumb_icon.png" /><p>當前位置：<a href="/">首頁(yè)</a> > <a href="/article.html">技術(shù)文章</a> > <span>硫化氫亞甲基藍分光光度法</span></p> </div>   <div id="bd8lmiie" class="ly_NyNewsdetail"> <h1 class="NyNewsdetail_title">硫化氫亞甲基藍分光光度法</h1> <div id="bd8lmiie" class="NyNewsdetail_tips">更新時(shí)間：2017-07-04 點(diǎn)擊次數：4445</div> <div id="bd8lmiie" class="NyNewsdetail_con"> <p><span style="font-size:14px"><strong>硫化氫：試劑的配置部分（</strong></span><strong>亞甲基藍分光光度法</strong><span style="font-size:14px"><strong>）</strong></span></p><p>1.<span style="font-size:10.5000pt"><span style="font-family:宋體">吸收液:4.3g硫酸鎘、0.3gNaOH和10g聚乙烯醇磷酸胺，分別溶于水后，混合，強烈振搖，混合均勻，定容到1000mL(乳白色懸浮液。在冰箱中可以保存一周).</span></span></p><p><span style="font-size:10.5000pt"><span style="font-family:宋體">2.三氯化鐵溶液:50g三氯化鐵（FeCl</span></span><sub><span style="font-size:10.5000pt"><span style="font-family:宋體">3</span></span></sub><span style="font-size:10.5000pt"><span style="font-family:宋體">·6 H</span></span><sub><span style="font-size:10.5000pt"><span style="font-family:宋體">2</span></span></sub><span style="font-size:10.5000pt"><span style="font-family:宋體">O），溶解于水中，稀釋到50mL(顯色輔助).</span></span></p><p><span style="font-size:10.5000pt"><span style="font-family:宋體">3.使用液:取50mL硫酸邊攪拌邊倒入50mL水，待冷。稱(chēng)取0.4g使用液（3號柜干燥器），溶于上述硫酸溶液中，加水至100mL。</span></span></p><p><span style="font-size:10.5000pt"><span style="font-family:宋體">4.混合顯色劑:臨用時(shí)，按1.00mL使用液和1滴三氧化鐵溶液混合(若溶液出現混濁，棄之，重配).</span></span></p><p><span style="font-size:10.5000pt"><span style="font-family:宋體">5.磷酸氫二銨溶液:稱(chēng)取20g磷酸氫二銨[(NH4)</span></span><sub><span style="font-size:10.5000pt"><span style="font-family:宋體"> 2</span></span></sub><span style="font-size:10.5000pt"><span style="font-family:宋體">·H PO</span></span><sub><span style="font-size:10.5000pt"><span style="font-family:宋體">4</span></span></sub><span style="font-size:10.5000pt"><span style="font-family:宋體">]，溶解于水中，稀釋到50mL(排除三價(jià)鐵的顏色).</span></span></p><p><span style="font-size:10.5000pt"><span style="font-family:宋體">6.</span></span><span style="font-size:12pt; line-height:1.6em"><span style="font-family:宋體">硫化鈉標準使用液（以S計）</span></span><span style="font-family:宋體; font-size:10.5pt; line-height:1.6em">10mg/L：</span><span style="font-family:宋體; font-size:12pt; line-height:1.6em">用100mg/L標準溶液配制，配200mL。</span></p><p><span style="font-size:12.0000pt"><span style="font-family:宋體">(A)配制250mL pH=10~12堿液（用NaoH顆粒調）；</span></span></p><p style="margin-left:21.6pt"><span style="font-size:12.0000pt"><span style="font-family:宋體">容量瓶中裝入一半的(A)，</span></span><span style="font-family:宋體; font-size:12pt; line-height:1.6em">加2mL乙酸鋅-乙酸鈉溶液，混勻；</span><span style="font-family:宋體; font-size:12pt; line-height:1.6em">取20mL標準溶液質(zhì)，邊振蕩邊滴入，以(A)定容，（</span><span style="font-family:宋體; font-size:10.5pt; line-height:1.6em">得到硫化鋅混懸液，使用前充分搖勻?？杀４姘肽辏?/span><span 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